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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
20/08/2018 |
Data da última atualização: |
22/08/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
NUNES, G. F. de O.; MENEZES, K. A. S.; SAMPAIO, A. A.; LEITE, J.; FERNANDES JUNIOR, P. I.; SEIDO, S. L.; ZILLI, J. E.; MARTINS, L. M. V. |
Afiliação: |
GERSIKA FAKIRRA DE OLIVEIRA NUNES, UNEB; KELLY ALEXSANDRA SOUZA MENEZES, UFAL; ALINE ARAÚJO SAMPAIO, UFBA; JAKSON LEITE, UFAL; PAULO IVAN FERNANDES JUNIOR, CPATSA; SIRANDO LIMA SEIDO, UFRPE; JERRI EDSON ZILLI, CNPAB; LINDETE MÍRIA VIEIRA MARTINS, UNEB. |
Título: |
Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Revista de Ciências Agrárias, Lisboa, v. 41, n. 3, p. 612-624, jul./set. 2018. |
DOI: |
http://dx.doi.org/10.19084/RCA17339 |
Idioma: |
Português |
Conteúdo: |
Forage legumes are important resources in semiarid regions due to their abilities to adapt to soils with low fertility and fix nitrogen when associated with diazotrophic bacteria. Here, we applied a polyphasic approach to characterize a set of legume nodule isolates obtained from Clitoria ternatea and Stylosllntlzes capitata cultivated in the soils of a semiarid region of Brazil. A tolal of 126 bacterial isolates were obtained: 45 isolates frorn C. ternatea and 81 isolates from S. capitata. Nodulation tests revealed only ten isolates that nodulated their original host: six isolates from C. iernaiea and four isolates from S. capitata. These ten legume nodule isolates were phenotypically and genotypically characterized. All isolates grew in fructose, glucose, sodium glutamate, maltose, xylose. and sucrose. Metabolic tests showed a relationship between tolerance to salt and high temperaturas, where isolates that tolerated the highest salt concentration also tolerated the highest ternperature. Three isolates showed amylolytic activity, and four isolates showed carboxymethyl cellulolytic activity. 5treptomycin was the most limiting and nalidixic acid was the least limiting antibiotic to bacterial growth. Seven out of ten isolates were indol-acetic acid producers. Additionally, 165 rRNA gene partial sequencing enabled the identification of members of the genera Bacillus (1), Bradvrhizobium (4), Leifeonia (3), Microvirga (1), and Rhizobium (1). These data reveal phenotypically and genotypically di verse bacteria inhabiting the nodules of the forage legumes C. tematea and S. capitata represent an important microbial source to protect new biotechnological products and improve forage legumes in semiarid regions. MenosForage legumes are important resources in semiarid regions due to their abilities to adapt to soils with low fertility and fix nitrogen when associated with diazotrophic bacteria. Here, we applied a polyphasic approach to characterize a set of legume nodule isolates obtained from Clitoria ternatea and Stylosllntlzes capitata cultivated in the soils of a semiarid region of Brazil. A tolal of 126 bacterial isolates were obtained: 45 isolates frorn C. ternatea and 81 isolates from S. capitata. Nodulation tests revealed only ten isolates that nodulated their original host: six isolates from C. iernaiea and four isolates from S. capitata. These ten legume nodule isolates were phenotypically and genotypically characterized. All isolates grew in fructose, glucose, sodium glutamate, maltose, xylose. and sucrose. Metabolic tests showed a relationship between tolerance to salt and high temperaturas, where isolates that tolerated the highest salt concentration also tolerated the highest ternperature. Three isolates showed amylolytic activity, and four isolates showed carboxymethyl cellulolytic activity. 5treptomycin was the most limiting and nalidixic acid was the least limiting antibiotic to bacterial growth. Seven out of ten isolates were indol-acetic acid producers. Additionally, 165 rRNA gene partial sequencing enabled the identification of members of the genera Bacillus (1), Bradvrhizobium (4), Leifeonia (3), Microvirga (1), and Rhizobium (1). These data reveal phenotypically and ... Mostrar Tudo |
Palavras-Chave: |
Fixação biológica do nitrogênio. |
Thesagro: |
Clitoria Ternatea; Controle Biológico; Fixação de Nitrogênio; Leguminosa Forrageira. |
Thesaurus Nal: |
Biological control; Bradyrhizobium; Forage legumes; Nitrogen fixation; Stylosanthes capitata. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/181656/1/RCA-vol41-n3-art04-RCA17339.pdf
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Marc: |
LEADER 02820naa a2200337 a 4500 001 2094257 005 2018-08-22 008 2018 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.19084/RCA17339$2DOI 100 1 $aNUNES, G. F. de O. 245 $aPolyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.$h[electronic resource] 260 $c2018 520 $aForage legumes are important resources in semiarid regions due to their abilities to adapt to soils with low fertility and fix nitrogen when associated with diazotrophic bacteria. Here, we applied a polyphasic approach to characterize a set of legume nodule isolates obtained from Clitoria ternatea and Stylosllntlzes capitata cultivated in the soils of a semiarid region of Brazil. A tolal of 126 bacterial isolates were obtained: 45 isolates frorn C. ternatea and 81 isolates from S. capitata. Nodulation tests revealed only ten isolates that nodulated their original host: six isolates from C. iernaiea and four isolates from S. capitata. These ten legume nodule isolates were phenotypically and genotypically characterized. All isolates grew in fructose, glucose, sodium glutamate, maltose, xylose. and sucrose. Metabolic tests showed a relationship between tolerance to salt and high temperaturas, where isolates that tolerated the highest salt concentration also tolerated the highest ternperature. Three isolates showed amylolytic activity, and four isolates showed carboxymethyl cellulolytic activity. 5treptomycin was the most limiting and nalidixic acid was the least limiting antibiotic to bacterial growth. Seven out of ten isolates were indol-acetic acid producers. Additionally, 165 rRNA gene partial sequencing enabled the identification of members of the genera Bacillus (1), Bradvrhizobium (4), Leifeonia (3), Microvirga (1), and Rhizobium (1). These data reveal phenotypically and genotypically di verse bacteria inhabiting the nodules of the forage legumes C. tematea and S. capitata represent an important microbial source to protect new biotechnological products and improve forage legumes in semiarid regions. 650 $aBiological control 650 $aBradyrhizobium 650 $aForage legumes 650 $aNitrogen fixation 650 $aStylosanthes capitata 650 $aClitoria Ternatea 650 $aControle Biológico 650 $aFixação de Nitrogênio 650 $aLeguminosa Forrageira 653 $aFixação biológica do nitrogênio 700 1 $aMENEZES, K. A. S. 700 1 $aSAMPAIO, A. A. 700 1 $aLEITE, J. 700 1 $aFERNANDES JUNIOR, P. I. 700 1 $aSEIDO, S. L. 700 1 $aZILLI, J. E. 700 1 $aMARTINS, L. M. V. 773 $tRevista de Ciências Agrárias, Lisboa$gv. 41, n. 3, p. 612-624, jul./set. 2018.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
25/03/2011 |
Data da última atualização: |
13/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ALZATE-MARIN, A. L.; NIETSCHE, S.; COSTA, M. R.; ALMEIDA, K. S. de; SARTORATO, A.; BARROS, E. G. de; MOREIRA, M. A. |
Afiliação: |
ANA LILIA ALZATE-MARIN, BIOAGRO; SILVA NIETSCHE, BIOAGRO; MARCIA REGINA COSTA, BIOAGRO; KRYSTYANO SOUZA DE ALMEIDA, BIOAGRO; ALOISIO SARTORATO, CNPAF; EVERALDO GONÇALVES DE BARROS, UFV; MAURILIO ALVES MOREIRA, UFV. |
Título: |
Molecular analyses of Colletotrichum lindemuthianum and Phaeoisariopsis griseola for pathotypes characterization. |
Ano de publicação: |
2001 |
Fonte/Imprenta: |
Annual Report of the Bean Improvement Cooperative, v. 44, p. 125-126, Mar. 2001. |
Idioma: |
Inglês |
Thesagro: |
Doença de planta; Feijão; Phaseolus vulgaris. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31144/1/BIC200111.pdf
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Marc: |
LEADER 00685naa a2200217 a 4500 001 1882943 005 2022-05-13 008 2001 bl uuuu u00u1 u #d 100 1 $aALZATE-MARIN, A. L. 245 $aMolecular analyses of Colletotrichum lindemuthianum and Phaeoisariopsis griseola for pathotypes characterization. 260 $c2001 650 $aDoença de planta 650 $aFeijão 650 $aPhaseolus vulgaris 700 1 $aNIETSCHE, S. 700 1 $aCOSTA, M. R. 700 1 $aALMEIDA, K. S. de 700 1 $aSARTORATO, A. 700 1 $aBARROS, E. G. de 700 1 $aMOREIRA, M. A. 773 $tAnnual Report of the Bean Improvement Cooperative$gv. 44, p. 125-126, Mar. 2001.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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